Intra cytoplasmic sperm injection (ICSI)
There are many variations on the IVF technique, and one that is particularly important concerns what happens where there are sperm present, but they are hardly swimming or not swimming at all, or there is some other barrier to fertilisation. The causes may be genetic or environmental. In normal IVF, the eggs and sperm are mixed in a petri dish and fertilisation occurs spontaneously. When the sperm are not active enough, this fertilisation does not happen. Fortunately ICSI was developed to account for this problem. By using sophisticated microscopic manipulation equipment, a single sperm can be manually injected into an egg resulting in fertilisation just as if a swimming sperm had encountered the egg naturally.
The procedure is called intra-cytoplasmic sperm injection (ICSI). Cytoplasm is just the scientific name for the inside of the egg. The other parts of the IVF cycle, the controlled ovarian stimulation and embryo transfer, are just the same as normal.
The ICSI Technique
Firstly, in ICSI, the egg and sperm are prepared separately. Then the egg is held in position by a pipette with gentle suction from a microinjector, with the polar body held away from the area to be injected. The embryologist then picks up the prepared sperm with a glass micropipette, pierces the shell of the egg and injects the sperm into the egg’s inner cytoplasm. The egg/sperm will then be placed in a culture medium, as normal, and checked the next day to see if it has fertilised. The egg and sperm are tiny and all this takes place using micromanipulators and a powerful microscope.